Analyses under the targeted lipomics lab are performed by extracting major membrane lipids from various tissues, plasma, cells, algae, bacterial extract, determining their fatty acid profile by gas chromatography, separating them into neutral and phospholipids and their corresponding sub fractions via thin layer chromatography, quantifying the amounts of these lipid classes and subclasses, determining their individual fatty acid compositions etc. Fatty acids containing ester bond from medium chain carbon 12 through carbon 26 having fully saturated carbon chain and unsaturated chain with different number and location of double bonds and commonly occurring ether bonds can be conveniently analyzed. Separation of cis/trans fatty acids, conjugated linoleic acid, lactobacilli acid of particular interest and related items are also performed with developed techniques. Quality control and reproducibility are checked to determine the analytical performances.
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